Signal Transduction Reagents and Kits
Enzo Life Sciences MMP-1 Colorimetric Assay Buffer (20ml)
Formulation: Liquid. In 50 mM HEPES, 10 mM calcium chloride, 0.05% Brij-35, 1 mM DTNB, pH 7.5. Long Term Storage: -20°C.
System Biosciences LLC pGreenFire1-ISRE (EF1α-neo) LentivectorpGreenFire1-ISRE (plasmid)+ EF1α-Neo= 10 µg
Study ISRE signal transduction by co-expressing dscGFP and luciferase in response to ISRE activity (includes EF1α-neo selection cassette). With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-ISRE (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from ISRE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of ISRE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to ISRE activity. The result is the ability to quantitatively measure ISRE activity by fluorescence and luciferase activity.
Carnabio Usa Inc MRCKA(CDC42BPA)/5UG/TRUNCATED
MRCK(CDC42BPA), Truncated protein, Wild type, Amino Acid 1-574, NP_003598.2, Expressed in Insect (sf21); 5microg
System Biosciences LLC TRANSDUX MAX LENTIVIRUS TRANSDUCTION REAGENTTransDux MAX Lentivirus Transduction Enhancer = 100 Transductions
Whether you’re working with difficult-to-transduce cells like primary T-cells or just want to ensure high transduction efficiency, SBI’s TransDux™ reagents are ready to deliver. Our newer TransDux MAX Lentiviral Transduction Reagent can increase transduction efficiencies by up to 8-fold compared to polybrene, while our original TransDux formulation (Cat# LV850A-1) is still available for researchers who are not yet ready to make the change.Ensure reliable and efficient gene delivery with TransDux MAX.
Neogen Diagnostics K-Blue Luminescent Substrate, 200 ML, HRP, Microwell, Chemiluminescent
K-Blue Luminescent is a stabilized luminescent substrate for use with horseradish peroxidase immunoassays. It contains both luminol and hydrogen peroxide (H2O2) in a two-bottle format with long-term stability, high sensitivity, and excellent signal to noise. Maximal light emission occurs at 430 nm in the presence of peroxidase labeled conjugate. K-Blue Luminescent is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. Stable for a minimum of 18-months when stored at 2-8C.
Medchemexpress LLC UK122 TFA | 940290-58-4 | 99.85% | 405.33 | 100 MG
UK122 is a potent and selective urokinase-type plasminogen activator (uPA) inhibitor with an IC50 of 0.2 μM. It exhibits minimal inhibition of tissue-type PA (tPA), plasmin, thrombin, and trypsin (all IC50 >100 μM). This 4-oxazolidinone analogue acts as an anticancer agent by inhibiting cancer cell migration and invasion.
- Dose-dependently inhibits cell migration
- Significantly reduces invasiveness of CFPAC-1 cells
- Does not affect cell growth or cell morphology in CFPAC-1 cells at certain concentrations
- Appears as a white to yellow solid
- Recommended for research use only
![CPC Scientific 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid 0.5MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00111436-SUBS-016.PNG-250.jpg)
CPC Scientific 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid 0.5MG
SEQUENCE: 4-(4-Dimethylaminophenylazo)benzoyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acidONE-LETTER SEQUENCE: 4-(4-Dimethylaminophenylazo)benzoyl-YVADAPV-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acidMOLECULAR FORMULA: C61H76N12O14S1MOLECULAR WEIGHT: 1233.39STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: [161877-70-9]SYNONYMS: Caspase 1 (ICE) Substrate; dabcyl-yvadapv-edans; dabcyl-tyr-val-ala-asp-ala-pro-val-edans; ICE Substrate Ii; il-1-beta Converting Enzyme Substrate Ii; il-1b Converting Enzyme Substrate Ii; 4-(4-dimethylaminophenylazo)benzoyl-tyr-val-ala-asp-ala-pro-val-5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid; 4-(4'-dimethylaminophenylazo)benzoyl-tyrosyl-valyl-alanyl-aspartyl-alanyl-prolyl-valyl-5((2-aminoethyl)amino)naphthalene-1-sulfonic acidRESEARCH AREA: ApoptosisREFERENCES:M.W. Pennington et al., Peptide Res., 7, 72 (1994)M. Los et al., Nature 375, 81 (1995)SKU(s): SUBS-016A, SUBS-016B
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 50 g dry blend (1 Liter prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 50 grams (reconstituted to 1 liter).
Medchemexpress LLC Tulobuterol | 41570-61-0 | MFCD00867022 | 99.7% | 100 MG
Tulobuterol is a long-acting β2-adrenoceptor agonist that helps reduce the frequency of exacerbations of chronic obstructive pulmonary disease and bronchial asthma. This sympathomimetic agent is also used as a transdermal patch, increasing normal diaphragm muscle strength. It is intended for research use only.
- Acts as a β2-adrenoceptor agonist
- Reduces RV14 RNA levels and sICAM-1 concentrations in infected cells
- Decreases acidic endosome numbers and fluorescence
- Inhibits RV14 infection-induced secretion of inflammatory cytokines (IL-1β, IL-6, and IL-8)
- Restores and maintains diaphragm muscle contractility
Montana Molecular Green Downward GENIe cGMP Sensor Kit D0800G, 30mL, CMV
Measure cyclic GMP (cGMP) signaling dynamics and ascertain the specificity of PDE inhibitors or SNP activators with this live cell fluorescent assay. Add GENIe to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cGMP increase. The assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. Kit includes soluble guanylate cyclase..